Monoclonal antibody to thyroid transcription factor-1: production, characterization, and usefulness in tumor diagnosis

Hybridoma. 1996 Feb;15(1):49-53. doi: 10.1089/hyb.1996.15.49.


Thyroid transcription factor-1 (TTF-1), a member of the NKx2 family of homeodomain transcription factors, is expressed in epithelial cells of the thyroid gland and the lung. To produce monoclonal antibodies specific for TTF-1, the polypeptide was expressed in E. coli and purified utilizing affinity chromatography of a polyhistidine-tagged TTF-1 fusion protein. Splenocytes from BALB/c mice immunized with recombinant TTF-1 were fused with P3x/63Ag8.653 myeloma cells to produce hybridomas. Tissue culture supernatant was screened for anti TTF-1 activity by ELISA employing recombinant TTF-1 as antigen. Hybridomas producing high-affinity antibodies were subcloned by limiting dilution. Antibodies from tissue culture fluid from an IgG1 clone (8G7G3/1) that stained the nuclei of paraffin-embedded human thyroid tissues were precipitation-purified and further characterized. The antibody stained a single 40-kDa polypeptide in immunoblots of nuclear extracts or lysates of cell lines known to express TTF-1 mRNA. MAb 8G7G3/1 also stained nuclei of tissue in a highly specific manner consistent with the pattern of expression obtained with an established polyclonal TTF-1 antibody and by in situ hybridization. MAb 8G7G3/1 was used for TTF-1 immunohistochemistry of human adenocarcinomas of the lung, colon, and breast as well as small cell carcinomas of the lung. TTF-1 was detected in primary lung adenocarcinomas and small cell carcinomas and was absent in colon and breast carcinomas. These findings demonstrate that anti-TTF-1 MAb 8G7G3/1 specifically binds TTF-1 in cell extracts and tissues and can be used to distinguish between lung and nonlung origin of a tumor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / diagnosis
  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry*
  • Biomarkers, Tumor / immunology*
  • Breast Neoplasms / diagnosis
  • Colonic Neoplasms / diagnosis
  • Female
  • Humans
  • Immunochemistry
  • Lung Neoplasms / diagnosis
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / genetics
  • Nuclear Proteins / immunology*
  • Recombinant Proteins / biosynthesis
  • Staining and Labeling
  • Thyroid Neoplasms / diagnosis
  • Thyroid Nuclear Factor 1
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcription Factors / immunology*


  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • NKX2-1 protein, human
  • Nkx2-1 protein, mouse
  • Nuclear Proteins
  • Recombinant Proteins
  • Thyroid Nuclear Factor 1
  • Transcription Factors