Cloning and Sequencing of the Gene Encoding the AmpC Beta-Lactamase of Morganella Morganii

FEMS Microbiol Lett. 1997 Mar 1;148(1):15-20. doi: 10.1111/j.1574-6968.1997.tb10260.x.

Abstract

The chromosomal beta-lactamase gene of a clinical isolate of Morganella morganii was cloned in Escherichia coli and sequenced. The beta-lactamase had a pI of 7.4 and conferred a typical AmpC susceptibility pattern. The insert obtained was found to encode a protein of 379 amino acids. Its deduced amino acid sequence revealed it to be a class C beta-lactamase: 39-56% identity with chromosomal AmpC beta-lactamases of Serratia marcescens, Yersinia enterocolitica, Citrobacter freundii, Enterobacter cloacae and Escherichia coli; and 37-56% identity with plasmid-mediated beta-lactamases (MOX-1, CMY-1, FOX-1, ACT-1, LAT-1, BIL-1 and CMY-2). The ampC gene was linked to a gene only part of which (450 bp) was cloned homologous to the regulatory ampR genes of chromosomal class C beta-lactamases.

MeSH terms

  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / analysis
  • Enterobacteriaceae / enzymology
  • Enterobacteriaceae / genetics*
  • Escherichia coli / genetics
  • Gene Expression Regulation, Bacterial / physiology
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transformation, Genetic
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • AmpC beta-lactamases
  • beta-Lactamases

Associated data

  • GENBANK/AF055067