Establishing a model to study the regulation of the lactose operon in Lactobacillus casei

FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x.


The chromosomally encoded lactose-specific phosphoenol pyruvate-dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15-] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15-] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Regulation, Bacterial / physiology
  • Genes, Bacterial / physiology
  • Genes, Reporter
  • Lac Operon / physiology*
  • Lacticaseibacillus casei / chemistry
  • Lacticaseibacillus casei / genetics*
  • Lacticaseibacillus casei / metabolism
  • Molecular Sequence Data
  • Mutation / physiology
  • Sequence Analysis, DNA
  • Transcription, Genetic / genetics*
  • beta-Galactosidase


  • beta-Galactosidase

Associated data

  • GENBANK/Z80834