Effects of interleukin-6 on in vitro cell attachment, migration and invasion of human ovarian carcinoma

Anticancer Res. Jan-Feb 1997;17(1A):337-42.

Abstract

High levels of IL-6 have been observed in ascitic fluid in patients with ovarian cancer. In this study, we examined the effects of IL-6 on in vitro invasiveness of human ovarian cancer cells. Human ovarian cancer cells, NOM1 and SKOV, were used. Cell attachment to Matrigel, migration, and invasion were examined with or without IL-6. Zymography was performed to analyze gelatinase production by these cells. NOM1 cell attachment was increased by IL-6 (0 to 10 ng/ml). In a migration assay, IL-6 ranging from 0 to 10 ng/ml showed chemotactic and/or chemokinetic activities for the two cell lines. The invasiveness of these cells was significantly enhanced by IL-6 at the above concentrations. However, IL-6 did not modulate the production of either MMPs or TIMPs. IL-6 affects cell invasion through its effects on attachment and migration in human ovarian cancer cells. IL-6 might thus contribute to the progression of this disease.

MeSH terms

  • Cell Adhesion / drug effects
  • Cell Movement / drug effects
  • Collagen
  • Drug Combinations
  • Female
  • Gelatinases / metabolism
  • Glycoproteins / biosynthesis
  • Humans
  • Interleukin-6 / pharmacology*
  • Laminin
  • Neoplasm Invasiveness
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology*
  • Protein Biosynthesis
  • Proteoglycans
  • Tissue Inhibitor of Metalloproteinase-2
  • Tissue Inhibitor of Metalloproteinases

Substances

  • Drug Combinations
  • Glycoproteins
  • Interleukin-6
  • Laminin
  • Proteoglycans
  • Tissue Inhibitor of Metalloproteinases
  • matrigel
  • Tissue Inhibitor of Metalloproteinase-2
  • Collagen
  • Gelatinases