Tyrosine hydroxylase activity and its mRNA level in dopaminergic neurons of tenascin gene knockout mouse

Biochem Biophys Res Commun. 1997 Feb 13;231(2):356-9. doi: 10.1006/bbrc.1997.6101.

Abstract

We have recently demonstrated that some tenascin (TN) gene-knockout mice display abnormal behaviors, and that these abnormal behaviors stem from a low level of dopamine transmission in the brain. In the present study, we elucidated that tyrosine hydroxylase (TH) activity in the frontal cortex, striatum, and hippocampus of TN-knockout mice which showed abnormal behavior was significantly decreased. Also, the TH mRNA level of the midbrain was decreased by 43% in these animals compared with values for wild-type mice. These results suggest that the low dopamine turnover rate in some areas of the brain of TN-knockout mice accompanied by motor defects is due, at least in part, to the reduction in TH activity caused by diminished TH mRNA expression, and that TN-knockout mice exhibit abnormal behaviors in the presence of low levels of TH-gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Dopamine / metabolism*
  • Mesencephalon / enzymology
  • Mice
  • Mice, Knockout
  • Motor Activity / genetics
  • Neurons / enzymology*
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Tenascin / genetics*
  • Tyrosine 3-Monooxygenase / genetics
  • Tyrosine 3-Monooxygenase / metabolism*

Substances

  • RNA, Messenger
  • Tenascin
  • Tyrosine 3-Monooxygenase
  • Dopamine