DJ-1, a Novel Oncogene Which Transforms Mouse NIH3T3 Cells in Cooperation With Ras

Biochem Biophys Res Commun. 1997 Feb 13;231(2):509-13. doi: 10.1006/bbrc.1997.6132.

Abstract

We have isolated and characterized the cDNA encoding a novel protein designated DJ-1. DJ-1, sharing no significant homology with the sequences so far reported, did not show transactivation activity in the Gal4 recombinant system, but transformed mouse NIH3T3 cells by itself. Furthermore, DJ-1 showed a cooperative transforming activity with H-Ras, more than 3 times as strong as the activity of ras/myc combination. DJ-1 was ubiquitously expressed in various human tissues, and the expression was induced by growth stimuli. Moreover, DJ-1 translocated from cytoplasm to nuclei in the S phase of the cell cycle. DJ-1 is thus suggested to be a novel mitogen-dependent oncogene product involved in a Ras-related signal transduction pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Cell Cycle / genetics
  • Cell Transformation, Neoplastic / genetics*
  • Cloning, Molecular
  • DNA, Complementary
  • Genes, ras*
  • HeLa Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Molecular Sequence Data
  • Oncogene Proteins / genetics*
  • Protein Deglycase DJ-1
  • Rats
  • Signal Transduction

Substances

  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Oncogene Proteins
  • PARK7 protein, human
  • Protein Deglycase DJ-1

Associated data

  • GENBANK/D61380