ADAM 13: a novel ADAM expressed in somitic mesoderm and neural crest cells during Xenopus laevis development

Dev Biol. 1997 Feb 15;182(2):314-30. doi: 10.1006/dbio.1996.8458.


Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They encode not only a potential adhesive function, through an integrin-binding disintegrin domain, but also a potential antiadhesive function, through a zinc-dependent metalloprotease domain. In order to investigate the role of ADAMs in early development we cloned a cDNA encoding a novel member of the ADAM family from a Xenopus laevis neurula stage library. We call this cDNA, and the 915-amino-acid protein it encodes, ADAM 13, X-ADAM 13 RNA is expressed during embryogenesis from the midblastula stage through tadpole stage 45. X-ADAM 13 is localized to somitic mesoderm and cranial neural crest cells during gastrulation, neurulation, and in tail bud stages. Sequence analyses of the X-ADAM 13 metalloprotease and disintegrin domains indicate that the protein is likely to be involved in both proteolytic and cell-adhesive functions. The X-ADAM 13 sequence is most closely related to that of mouse meltrin alpha, which is implicated in myoblast fusion. Our data suggest that X-ADAM 13 may be involved in neural crest cell adhesion and migration as well as myoblast differentiation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • ADAM Proteins
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • DNA, Complementary
  • Disintegrins / genetics
  • Disintegrins / physiology
  • Embryo, Nonmammalian / metabolism
  • Humans
  • Mammals
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / physiology
  • Mice
  • Molecular Sequence Data
  • Neural Crest / cytology
  • Neural Crest / metabolism*
  • RNA, Messenger / biosynthesis
  • Sequence Homology, Amino Acid
  • Somites / metabolism*
  • Xenopus Proteins*
  • Xenopus laevis / embryology


  • DNA, Complementary
  • Disintegrins
  • Membrane Proteins
  • RNA, Messenger
  • Xenopus Proteins
  • ADAM Proteins
  • ADAM33 protein, Xenopus
  • Metalloendopeptidases

Associated data

  • GENBANK/U66003