Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2

Biochem Biophys Res Commun. 1997 Feb 24;231(3):523-5. doi: 10.1006/bbrc.1997.6089.


To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Carnitine O-Palmitoyltransferase / metabolism*
  • Intracellular Membranes / metabolism
  • Membrane Proteins / metabolism*
  • Mice
  • Mitochondria / metabolism*
  • Precipitin Tests
  • Protein Binding
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Recombinant Proteins
  • Structure-Activity Relationship


  • Membrane Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Proteins
  • Carnitine O-Palmitoyltransferase