Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescence in Situ Hybridization

J Histochem Cytochem. 1997 Mar;45(3):375-82. doi: 10.1177/002215549704500305.

Abstract

The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluorescence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions, all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISH methods.

MeSH terms

  • Animals
  • Antigens / analysis
  • Fluorescent Dyes / chemistry*
  • Horseradish Peroxidase
  • Humans
  • Immunoenzyme Techniques
  • Immunohistochemistry / methods*
  • In Situ Hybridization, Fluorescence / methods*
  • Rats
  • Sensitivity and Specificity
  • Tyramine / chemistry*
  • Vimentin / analysis

Substances

  • Antigens
  • Fluorescent Dyes
  • Vimentin
  • Horseradish Peroxidase
  • Tyramine