Spermidine biosynthesis in Saccharomyces cerevisae: polyamine requirement of a null mutant of the SPE3 gene (spermidine synthase)

Gene. 1997 Mar 10;187(1):35-43. doi: 10.1016/s0378-1119(96)00660-9.


The Saccharomyces cerevisiae SPE3 gene, coding for spermidine synthase, was cloned, sequenced, and localized on the right arm of chromosome XVI. The deduced amino acid sequence has a high similarity to mammalian spermidine synthases, and has putative S-adenosylmethionine binding motifs. To investigate the effect of total loss of the SPE3 gene, we constructed a null mutant of this gene, spe3delta, which has no spermidine synthase activity and has an absolute requirement for spermidine or spermine for the growth. This requirement is satisfied by a very low concentration of spermidine (10(-8) M) or a higher concentration of spermine (10(-6) M).

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Conserved Sequence
  • Deoxyadenosines / metabolism
  • Genes, Fungal*
  • Molecular Sequence Data
  • Mutation
  • S-Adenosylmethionine / analogs & derivatives
  • S-Adenosylmethionine / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Spermidine / biosynthesis*
  • Spermidine / metabolism
  • Spermidine Synthase / genetics*
  • Thionucleosides / metabolism


  • Deoxyadenosines
  • Thionucleosides
  • S-adenosyl-3-methylthiopropylamine
  • 5'-methylthioadenosine
  • S-Adenosylmethionine
  • Spermidine Synthase
  • Spermidine

Associated data

  • GENBANK/U27519