Cloning of the human twist gene: its expression is retained in adult mesodermally-derived tissues

Gene. 1997 Mar 10;187(1):83-92.

Abstract

We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Conserved Sequence
  • Fibroblasts
  • Gene Expression Regulation*
  • Helix-Loop-Helix Motifs*
  • Humans
  • Mesoderm / metabolism*
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Promoter Regions, Genetic / genetics
  • Restriction Mapping
  • Sequence Analysis
  • Single-Strand Specific DNA and RNA Endonucleases / metabolism
  • Species Specificity
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription, Genetic
  • Twist-Related Protein 1

Substances

  • Nuclear Proteins
  • TWIST1 protein, human
  • Transcription Factors
  • Twist-Related Protein 1
  • Single-Strand Specific DNA and RNA Endonucleases

Associated data

  • GENBANK/X91662