Antisense transcription of a murine FGFR-3 psuedogene during fetal developement

Gene. 1997 Mar 10;187(1):115-22. doi: 10.1016/s0378-1119(96)00733-0.

Abstract

In a search for new protein tyrosine kinases (PTKs) in early hemopoietic cells, we have identified a sequence closely related to the Fibroblast Growth Factor Receptor (FGFR) family. A cDNA isolated from a mouse embryo library was 89% identical to FGFR-3 in both its coding and 3' untranslated regions. However, the region homologous to exons 5 to 9 of FGFR-3 was missing. In addition, the ORF was interrupted by several stop codons and frame shifts, indicating that this sequence is not functional. These transcripts were therefore copied from a novel FGFR-3 pseudogene, that we called psiFGFR-3. Partial analysis of this gene showed the absence of introns, which is a characteristic feature of a processed pseudogene. psiFGFR-3 gene was localized on Chromosome 1H4-6. Its transcription was shown to be antisense and its expression was restricted to fetal tissues. These results indicate that psiFGFR-3 has been inserted in Chromosome 1 in antisense orientation close to a heterologous promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Embryo, Mammalian / metabolism
  • Fibroblast Growth Factors
  • Gene Expression Regulation, Developmental*
  • Introns / genetics
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein-Tyrosine Kinases / chemistry
  • Pseudogenes / genetics*
  • RNA, Antisense / genetics*
  • Receptor, Fibroblast Growth Factor, Type 3
  • Receptors, Fibroblast Growth Factor / chemistry
  • Receptors, Fibroblast Growth Factor / genetics*
  • Sequence Analysis
  • Sequence Deletion / genetics
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*

Substances

  • RNA, Antisense
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factors
  • Fgfr3 protein, mouse
  • Protein-Tyrosine Kinases
  • Receptor, Fibroblast Growth Factor, Type 3

Associated data

  • GENBANK/U52463