Abstract
The cDNA coding for GT3-synthase has been cloned from a rat fetal brain cDNA library. The complete nucleotide sequence and the deduced amino acid sequence of the rat GT3-synthase cDNA were highly homologous to those of the mouse GT3-synthase. Quantitative RT-PCR showed that, as a key enzyme for the synthesis of 'c'-series gangliosides, the expression of GT3-synthase was developmentally regulated in embryonic rat brains.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Brain / embryology
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Brain / enzymology*
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Cloning, Molecular
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Conserved Sequence / genetics
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DNA Primers
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation, Developmental*
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Membrane Proteins / chemistry
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Molecular Sequence Data
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Polymerase Chain Reaction
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Rats
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Sialyltransferases / chemistry*
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Sialyltransferases / genetics*
Substances
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DNA Primers
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DNA, Complementary
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Membrane Proteins
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Sialyltransferases
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alpha-N-acetylneuraminate alpha-2,8-sialyltransferase