Isolation, extraction, and measurement of acetylcholine from Lactobacillus plantarum

Appl Environ Microbiol. 1977 Aug;34(2):237-9. doi: 10.1128/AEM.34.2.237-239.1977.

Abstract

The isolation, extraction, and spectrophotometric determination of acetylcholine from Lactobacillus plantarum ATCC 10241 is described. Acetylcholine was extracted with a mixture of sodium tetraphenylboron-butylethylketone-acetonitrile and was measured enzymatically at 340 nm.

MeSH terms

  • Acetonitriles
  • Acetylcholine / analysis
  • Acetylcholine / isolation & purification*
  • Ketones
  • Lactobacillus / analysis*
  • Spectrophotometry / methods
  • Tetraphenylborate

Substances

  • Acetonitriles
  • Ketones
  • Tetraphenylborate
  • Acetylcholine