Pre-activating wounding response in tobacco prior to high-level ozone exposure prevents necrotic injury

Plant J. 1997 Feb;11(2):203-12. doi: 10.1046/j.1365-313x.1997.11020203.x.

Abstract

In tobacco, both wounding and treatment with jasmonates prior to exposure of the tissue to high concentrations of ozone (250 to 500 p.p.b.) produce a dramatic decrease in ozone injury. A systemic pattern of increased ozone tolerance developed within 3-6 h after wounding and also after local application of jasmonates. Ozone treatment of transgenic (NahG) tobacco plants showed that the inability of these plants to accumulate salicylic acid is also accompanied by increased ozone tolerance. Expression of mRNA encoding the anti-oxidant enzyme ascorbate peroxidase is upregulated by ozone challenge, wounding and by methyl jasmonate exposure within 3-4 h, while levels of carbonic anhydrase mRNA are simultaneously depressed following ozone exposure and methyl jasmonate treatment. The pattern of these results shows that the response to ozone challenge in tobacco involves signalling mechanisms similar to those induced in plants by other environmental stresses that generate reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / pharmacology
  • Air Pollutants / pharmacology*
  • Ascorbate Peroxidases
  • Carbonic Anhydrases / genetics
  • Catalase / genetics
  • Cyclopentanes / pharmacology
  • Gene Expression Regulation, Plant / drug effects
  • Mixed Function Oxygenases / genetics
  • Oxylipins
  • Ozone / pharmacology*
  • Peroxidases / genetics
  • Plant Growth Regulators / pharmacology
  • Plants, Genetically Modified
  • Plants, Toxic*
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Salicylates / metabolism
  • Salicylic Acid
  • Tobacco / drug effects*
  • Tobacco / physiology

Substances

  • Acetates
  • Air Pollutants
  • Cyclopentanes
  • Oxylipins
  • Plant Growth Regulators
  • RNA, Messenger
  • RNA, Plant
  • Salicylates
  • Ozone
  • methyl jasmonate
  • Mixed Function Oxygenases
  • Peroxidases
  • Ascorbate Peroxidases
  • Catalase
  • salicylate 1-monooxygenase
  • Carbonic Anhydrases
  • Salicylic Acid