Identification of an Arabidopsis locus required for resistance to turnip crinkle virus

Plant J. 1997 Feb;11(2):301-11. doi: 10.1046/j.1365-313x.1997.11020301.x.

Abstract

Inoculation of turnip crinkle virus (TCV) into a (TCV)-resistant line of Arabidopsis thaliana, Di-17, results in the development of a hypersensitive response (HR) on the inoculated leaves. In contrast, an HR does not occur when leaves of the TCV-susceptible Di-3 line or the susceptible ecotypes Columbia (Col-0), or Landsberg erecta (Ler) are inoculated. Genetic analysis of progeny from crosses between Di-17 and either Di-3, Col-0 or Ler demonstrates that the development of an HR is regulated by a single dominant nuclear locus, herein designated HRT. Using progeny from a Di-17 x Col-0 cross, HRT was mapped to chromosome 5, where it is tightly linked to the DFR locus. We also demonstrate that a variety of resistance-associated phenomena, including the TCV-induced accumulation of salicylic acid, camalexin and autofluorescent cell-wall material, correlate with the HR, suggesting the possibility that HRT is required for their activation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / physiology
  • Arabidopsis / virology
  • Carmovirus / growth & development*
  • Cell Wall / chemistry
  • Chromosome Mapping
  • Crosses, Genetic
  • Genes, Plant / physiology*
  • Indoles / analysis
  • Plant Diseases* / virology
  • Plant Proteins / analysis
  • Salicylates / analysis
  • Salicylic Acid
  • Thiazoles / analysis

Substances

  • Indoles
  • Plant Proteins
  • Salicylates
  • Thiazoles
  • camalexin
  • Salicylic Acid