Assay for the determination of urinary 8-hydroxy-2'-deoxyguanosine by high-performance liquid chromatography with electrochemical detection

J Chromatogr B Biomed Sci Appl. 1997 Feb 21;689(2):399-403. doi: 10.1016/s0378-4347(96)00328-3.


A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Humans


  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine