Autotrophic carbon dioxide fixation in Acidianus brierleyi

Arch Microbiol. 1996 Dec;166(6):368-71. doi: 10.1007/BF01682981.

Abstract

The autotrophic CO2 fixation pathway in Acidianus brierleyi, a facultatively anaerobic thermoacidophilic archaebacterium, was investigated by measuring enzymatic activities from autotrophic, mixotrophic, and heterotrophic cultures. Contrary to the published report that the reductive tricarboxylic acid cycle operates in A. brierleyi, the enzymatic activity of ATP:citrate lyase, the key enzyme of the cycle, was not detected. Instead, activities of acetyl-CoA carboxylase and propionyl-CoA carboxylase, key enzymes of the 3-hydroxypropionate cycle, were detected only when A. brierleyi was growing autotrophically. We conclude that a modified 3-hydroxypropionate pathway operates in A. brierleyi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / metabolism
  • Adenosine Triphosphate / metabolism
  • Anaerobiosis
  • Carbon Dioxide / metabolism*
  • Carboxy-Lyases / metabolism
  • Citric Acid Cycle
  • Culture Media / chemistry
  • Culture Media / metabolism
  • Lactic Acid / analogs & derivatives
  • Lactic Acid / metabolism
  • Methylmalonyl-CoA Decarboxylase
  • Multienzyme Complexes / metabolism
  • Oxo-Acid-Lyases / metabolism
  • Sulfolobaceae / enzymology*
  • Sulfolobaceae / growth & development
  • Sulfolobaceae / metabolism*

Substances

  • Culture Media
  • Multienzyme Complexes
  • Carbon Dioxide
  • Lactic Acid
  • Adenosine Triphosphate
  • hydracrylic acid
  • Carboxy-Lyases
  • Oxo-Acid-Lyases
  • citrate (pro-3S)-lyase
  • Acetyl-CoA Carboxylase
  • Methylmalonyl-CoA Decarboxylase