Bradykinin B1 receptors in human umbilical vein

Eur J Pharmacol. 1997 Feb 19;321(1):33-8. doi: 10.1016/s0014-2999(96)00927-2.


The present study was undertaken to demonstrate the presence of bradykinin B1 receptors mediating contraction of human umbilical vein. The bradykinin B1 receptor selective agonist, des-Arg9-bradykinin, produced a dose-dependent contractile response of human umbilical vein rings. Furthermore, des-Arga-bradykinin-mediated response increased in a time-dependent manner in vitro. The maximal response to des-Arg9-bradykinin, expressed as percentage of the maximum elicited by serotonin, was: 10 +/- 2 at 15 min, 55 +/- 5 at 120 min and 80 +/- 3 at 300 min. Des-Arg9-bradykinin-mediated contractions were inhibited by the specific bradykinin B1 receptor antagonist des-Arg9-[Leu8]bradykinin which produced parallel shifts in the dose-response curve to the selective bradykinin B1 receptor agonist. Schild regression analysis of data established a pA2 value of 6.16 +/- 0.06. Kinin-induced contraction was not modified by pre-treatment with indomethacin (10 microM), a cyclo-oxygenase inhibitor. On the other hand, continuous exposure to the anti-inflammatory steroid dexamethasone (100 microM) or to the protein synthesis inhibitor cycloheximide (70 microM) largely prevented the sensitization to des-Arg9-bradykinin in incubated human umbilical vein rings. These results confirm the presence of bradykinin B1 receptors which mediate contraction in isolated human umbilical vein. These responses are up-regulated in a time- and protein synthesis-dependent process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology
  • Bradykinin / analogs & derivatives
  • Bradykinin / pharmacology
  • Bradykinin Receptor Antagonists
  • Cyclooxygenase Inhibitors / pharmacology
  • Dexamethasone / pharmacology
  • Female
  • Humans
  • In Vitro Techniques
  • Muscle Contraction / physiology
  • Pregnancy
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin / agonists
  • Receptors, Bradykinin / drug effects*
  • Umbilical Veins / drug effects*
  • Up-Regulation / drug effects


  • Anti-Inflammatory Agents
  • Bradykinin Receptor Antagonists
  • Cyclooxygenase Inhibitors
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin
  • bradykinin, des-Arg(9)-
  • bradykinin, Leu(8)-des-Arg(9)-
  • icatibant
  • Dexamethasone
  • Bradykinin