Roles of Cartilage Matrix Proteins, chondromodulin-I and -II, in Endochondral Bone Formation: A Review

Connect Tissue Res. 1996;35(1-4):303-7. doi: 10.3109/03008209609029204.


Insulin induces early chondrogenesis in cultures of a clonal cell-line, ATDC5, which was derived from mouse embryonal carcinoma line AT805. Cartilage-generated matrix components chondromodulin-I (ChM-I) synergistically stimulates growth and differentiation of chondrocytes in the presence or absence of FGF-2. In contrast, ChM-I inhibits the proliferation of vascular endothelial cells and tube formation, thereby further stimulating cartilage growth and inhibiting replacing cartilage by bone in an early stage. Another cartilage-derived chondromodulin-II (ChM-II) also stimulates cartilage growth. However, ChM-II does not inhibit vascularization but stimulates osteoclast differentiation. Therefore, endochondral bone formation is regulated sequentially by cartilage-derived multiple autocrine factors. This opens a new mechanism of regulation of endochondral bone formation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cartilage
  • Cell Differentiation
  • Cells, Cultured
  • Extracellular Matrix Proteins*
  • Glycoproteins / physiology
  • Growth Plate / cytology*
  • Growth Plate / metabolism*
  • Growth Substances / physiology*
  • Intercellular Signaling Peptides and Proteins*
  • Matrilin Proteins
  • Membrane Proteins*
  • Molecular Sequence Data
  • Osteogenesis*
  • Proteins / physiology*


  • Cnmd protein, mouse
  • Extracellular Matrix Proteins
  • Glycoproteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Lect2 protein, mouse
  • Matn1 protein, mouse
  • Matrilin Proteins
  • Membrane Proteins
  • Proteins