Enzyme activities in quadriceps femoris muscle of obese diabetic male patients

Diabetologia. 1977 Sep;13(5):527-9. doi: 10.1007/BF01234508.

Abstract

In biopsy samples of the lateral part of the quadriceps femoris muscle of 6 obese diabetic male patients and of 11 obese males with a normal glucose tolerance, the activities of 7 enzymes of energy metabolism were estimated: hexokinase, cytoplasmic glycerol-3-phosphate: NAD dehydrogenase, triosephosphate dehydrogenase, lactate dehydrogenase, citrate synthase, malate dehydrogenase and 3-hydroxyacyl-CoA dehydrogenase. The obese diabetic male patients exhibited decreased activities of enzymes of carbohydrate breakdown and cytoplasmic NAD regeneration. Enzymes connected functionally with aerobic metabolism were less affected. The unchanged activity of 3-hydroxyacyl-CoA dehydrogenase points to an increased role of fatty acid catabolism in the muscle.

Publication types

  • Comparative Study

MeSH terms

  • 3-Hydroxyacyl CoA Dehydrogenases / metabolism
  • Citrate (si)-Synthase / metabolism
  • Diabetes Mellitus / enzymology*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Glycerolphosphate Dehydrogenase / metabolism
  • Hexokinase / metabolism
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Malate Dehydrogenase / metabolism
  • Male
  • Muscles / enzymology*
  • Obesity / enzymology

Substances

  • Glycerolphosphate Dehydrogenase
  • 3-Hydroxyacyl CoA Dehydrogenases
  • L-Lactate Dehydrogenase
  • Malate Dehydrogenase
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Citrate (si)-Synthase
  • Hexokinase