Interleukin-7-enhanced cytotoxic T lymphocyte activity after viral infection in marrow transplanted mice

Bone Marrow Transplant. 1997 Mar;19(6):539-43. doi: 10.1038/sj.bmt.1700706.

Abstract

Lethally irradiated BALB/c mice were reconstituted by syngeneic bone marrow transplantation (BMT), and injected with recombinant interleukin 7 (rIL-7), recombinant interleukin 2 (rIL-2), or saline 10 days post-transplantation. Intranasal infection with A/PR8/34 influenza virus 2 weeks after BMT was associated with the highest survival rate in the rIL-7 treated group. The protective mechanism elicited by rIL-7, as manifested by very low virus titers in the lung, involves T and B cell functions. High hemagglutinin inhibition antibody levels were observed on days 7 and 12 post-challenge in the rIL-7 mice. Moreover, the anti-influenza cytotoxic T lymphocyte activity was induced primarily by rIL-7, leaving the effect of rIL-2 on the same level as that of the control. Thus, rIL-7 promotes both T cell-mediated function and B cell production during the immunodeficient state after BMT. This cytokine may prove a potential immunotherapeutic modality in BMT recipients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Transplantation* / adverse effects
  • Bone Marrow Transplantation* / immunology
  • Cytotoxicity, Immunologic / drug effects*
  • Female
  • Interleukin-7 / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections / immunology*
  • Orthomyxoviridae*
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes / immunology*
  • Transplantation, Isogeneic

Substances

  • Interleukin-7
  • Recombinant Proteins