Discovery of Disseminated J96-like Strains of Uropathogenic Escherichia Coli O4:H5 Containing Genes for Both PapG(J96) (Class I) and PrsG(J96) (Class III) Gal(alpha1-4)Gal-binding Adhesins

J Infect Dis. 1997 Apr;175(4):983-8. doi: 10.1086/514006.

Abstract

The pyelonephritis-associated adhesin gene papG of Escherichia coli occurs in three variants. Whereas the class II and class III variants are common among human urinary tract infection isolates, the class I allele, despite being the first cloned, has previously been found only in source strain J96. Five strains have been discovered from geographically diverse locales that, like J96, contain both the class I and class III papG alleles. One strain caused bacteremia, whereas 4 caused cystitis. Like J96, all 5 had group III capsule genes, expressed the H5 flagellar antigen and the F13 fimbrial antigen, and exhibited similar genomic patterns and virulence factor profiles. These findings demonstrate that the class I papG allele is not unique to J96 but is present in a group of extraintestinal isolates of E. coli O4:H5 that represent a disseminated virulent clonal group.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adhesins, Escherichia coli / genetics*
  • Alleles
  • Blotting, Southern
  • Disaccharides / metabolism*
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli / classification*
  • Escherichia coli / genetics
  • Fimbriae Proteins*
  • Genes, Bacterial*
  • Humans
  • Serotyping
  • Urinary Tract Infections / microbiology*
  • Virulence

Substances

  • Adhesins, Escherichia coli
  • Disaccharides
  • PapG protein, E coli
  • galactosyl-(1-3)galactose
  • Fimbriae Proteins