Reverse transcriptase-like activity in Trypanosoma cruzi

Acta Trop. 1997 Feb;63(2-3):117-26. doi: 10.1016/s0001-706x(96)00611-0.

Abstract

The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/ oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg+2 ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D. novobiocin, rifamycin SV and AZT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin / pharmacology
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Dactinomycin / pharmacology
  • Dideoxynucleotides
  • Enzyme Inhibitors / pharmacology
  • Magnesium / metabolism
  • Nucleic Acid Synthesis Inhibitors / pharmacology
  • Poly A / genetics
  • Poly C / genetics
  • Poly G / genetics
  • Poly T / genetics
  • RNA, Protozoan / genetics
  • RNA-Directed DNA Polymerase / metabolism*
  • Reverse Transcriptase Inhibitors / pharmacology
  • Rifamycins / pharmacology
  • Thymine Nucleotides / pharmacology
  • Transcription, Genetic*
  • Trypanosoma / genetics*
  • Zidovudine / pharmacology

Substances

  • Dideoxynucleotides
  • Enzyme Inhibitors
  • Nucleic Acid Synthesis Inhibitors
  • RNA, Protozoan
  • Reverse Transcriptase Inhibitors
  • Rifamycins
  • Thymine Nucleotides
  • Dactinomycin
  • Poly A
  • Poly T
  • Poly G
  • poly G-poly C
  • poly A-T
  • Poly C
  • Aphidicolin
  • Zidovudine
  • rifamycin SV
  • RNA-Directed DNA Polymerase
  • Magnesium
  • 2',3'-dideoxythymidine triphosphate