Heterogeneity of expression of CD68 and other macrophage-associated antigens in human long-term bone marrow culture

Biologicals. 1996 Dec;24(4):333-7. doi: 10.1006/biol.1996.0047.

Abstract

The authors have performed an immunohistochemical study of intact human long-term bone marrow cultures (hLTBMC) grown directly onto glass slides. Between 4 and 12 weeks of growth, such cultures consist of a complex stromal layer supporting foci of haemopoietic cells which undergo granulocytic and monocytic differentiation. As part of a large panel of antibodies employed to characterize monocytes and macrophages within hLTBMC, we included six different anti-CD68 reagents and three antibodies representing a putative new CD group recognizing a macrophage-associated antigen of 130 kDa molecular weight. These gave heterogeneous immunostaining patterns with macrophages and stromal myofibroblastic cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Antigens, CD / biosynthesis*
  • Antigens, CD / immunology
  • Antigens, Differentiation, Myelomonocytic / biosynthesis*
  • Antigens, Differentiation, Myelomonocytic / immunology
  • Bone Marrow Cells*
  • Cell Differentiation
  • Cell Lineage
  • Cells, Cultured
  • Epitopes / immunology
  • Hematopoietic Stem Cells / cytology*
  • Humans
  • Macrophages / cytology*
  • Macrophages / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD68 antigen, human
  • Epitopes