Large-scale production of class I bound peptides: assigning a signature to HLA-B*1501

Immunogenetics. 1997;45(6):379-85. doi: 10.1007/s002510050219.

Abstract

A peptide-based vaccine must be bound and presented by major histocompatibility complex class I molecules to elicit a CD8(+) T-cell response. Because class I HLA molecules are highly polymorphic, it has yet to be established how well a vaccine peptide that stimulates one individual's CD8(+) cytotoxic T lymphocytes will be presented by a second individual's different class I molecules. Therefore, to facilitate precise comparisons of class I peptide binding overlaps, we uniquely combined hollow-fiber bioreactors and mass spectrometry to assign precise peptide binding signatures to individual class I HLA molecules. In applying this strategy to HLA-B*1501, we isolated milligram quantities of B*1501-bound peptides and mapped them using mass spectrometry. Repeated analyses consistently assign the same peptide binding signature to B*1501; the degree of peptide binding overlap between any two class I molecules can thus be determined through comparison of their peptide signatures.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cytotoxicity, Immunologic
  • HLA-B Antigens / immunology*
  • Humans
  • Peptides / chemistry
  • Peptides / immunology*
  • Peptides / metabolism
  • Protein Binding
  • Vaccines, Synthetic / immunology*

Substances

  • HLA-B Antigens
  • Peptides
  • Vaccines, Synthetic