Murine colon adenocarcinoma: syngeneic orthotopic transplantation and subsequent hepatic metastases

J Natl Cancer Inst. 1977 Nov;59(5):1537-44. doi: 10.1093/jnci/59.5.1537.


Syngeneic murine colon adenocarcinoma (MCA-38) cells were transplanted in the submucosa of distal colon, proximal colon, cecum, ileum, jejunum, and duodenum of male C57BL/6 mice, with local lymphoid follicles used as points of entry. The tumor grew best at the cecum and led to liver and mesenteric lymph node metastases in 8 and 9 weeks, respectively, after transplantation. Histologically, a local inflammatory reaction involving polymorphonuclear leukocytes was observed within 48-72 hours following transplantation; after this time, the microscopic tumor foci began to grow progressively. Mononuclear lymphoid cells of the gut-associated lymphoid tissue did not infiltrate the progressively growing tumor; however, polymorphonuclear leukocytes were constantly observed at the tumor periphery in the lamina propria. The studies indicated that orthotopic transplantation as a model system can provide a means of examining the role of the local immune response as a focus of host resistance and as a factor in metastatic tumor spread. The findings also suggested the usefulness of this model in immunotherapeutic and chemotherapeutic studies of secondary hepatic disease.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / pathology*
  • Animals
  • Cecal Neoplasms / pathology
  • Colonic Neoplasms / pathology*
  • Duodenal Neoplasms / pathology
  • Ileum
  • Intestinal Neoplasms / pathology
  • Jejunum
  • Liver Neoplasms / pathology*
  • Lymphatic Metastasis
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Metastasis
  • Neoplasm Transplantation
  • Neutrophils / pathology
  • Transplantation, Isogeneic