B lymphocyte-specific, Cre-mediated mutagenesis in mice

Nucleic Acids Res. 1997 Mar 15;25(6):1317-8. doi: 10.1093/nar/25.6.1317.


Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD19 / biosynthesis
  • Antigens, CD19 / genetics*
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Blotting, Southern
  • Bone Marrow
  • Crosses, Genetic
  • DNA Primers
  • Gene Deletion*
  • Genetic Techniques
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology
  • Introns
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Mutagenesis*
  • Polymerase Chain Reaction / methods
  • Recombination, Genetic
  • Spleen / cytology
  • Spleen / immunology


  • Antigens, CD19
  • DNA Primers