Identification of PRG1, a novel progestin-responsive gene with sequence homology to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Mol Endocrinol. 1997 Apr;11(4):490-502. doi: 10.1210/mend.11.4.9909.


To define early molecular targets of progestin action, the differential display technique was used to identify genes with altered levels of expression in T-47D breast cancer cells treated with the synthetic progestin ORG 2058 for 3 h. PRG1 was first isolated as a 200-bp cDNA clone and its progestin regulation confirmed by Northern analysis. Cloning of the complete coding region of PRG1 revealed that it shared a high degree of amino acid sequence identity with isoforms of the enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from several tissues and species. Expression of PRG1 mRNA was observed in several normal breast epithelial and breast cancer cell lines and in a variety of human tissues, with highest expression in the breast, aorta, and brain. In T-47D cells, PRG1 mRNA was rapidly and transiently induced by progestins, expression peaking between 2 and 4 h and returning to control levels by 12 h. Progestin-induced increases in PRG1 mRNA were inhibited by the progestin antagonist RU 486 and occurred via the progesterone receptor. Progestin induction of PRG1 mRNA was also inhibited by actinomycin D but not by cycloheximide. PRG1 is therefore a novel human gene that is directly regulated by progestins via the progesterone receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Breast Neoplasms / enzymology
  • Breast Neoplasms / genetics
  • Cattle
  • Cell Cycle / genetics
  • Cloning, Molecular
  • DNA, Complementary
  • Female
  • Fructose-Bisphosphatase / chemistry*
  • Fructose-Bisphosphatase / genetics
  • Gene Expression Regulation
  • Glycolysis / genetics
  • Humans
  • Molecular Sequence Data
  • Phosphofructokinase-1 / chemistry*
  • Phosphofructokinase-1 / genetics
  • Phosphofructokinase-2
  • Pregnenediones / pharmacology
  • Progesterone Congeners / pharmacology
  • Progestins / physiology*
  • Protein Synthesis Inhibitors / pharmacology
  • Proteins / chemistry
  • Proteins / genetics*
  • RNA, Messenger / isolation & purification
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • DNA, Complementary
  • Pregnenediones
  • Progesterone Congeners
  • Progestins
  • Protein Synthesis Inhibitors
  • Proteins
  • RNA, Messenger
  • 16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione
  • PFKFB3 protein, human
  • Phosphofructokinase-2
  • Phosphofructokinase-1
  • Fructose-Bisphosphatase

Associated data

  • GENBANK/L77662