In vivo proton magnetic resonance spectroscopy for metabolic changes in brain during chronic cerebral vasospasm in primates

Neurosurgery. 1997 Apr;40(4):773-80; discussion 780-1. doi: 10.1097/00006123-199704000-00023.


Objective: To study how neuronal cells are affected by development of chronic cerebral vasospasm after subarachnoid hemorrhage (SAH), the changes in neuronal metabolites during development of vasospasm were evaluated by in vivo localized proton magnetic resonance spectroscopy (MRS) in primates.

Methods: SAH was produced by introduction of a blood clot around the right middle cerebral artery and the right side of the circle of Willis. MRS experiments were performed before SAH and on Days 7 and 14 after SAH. Multislice magnetic resonance images were obtained to locate the volume of interest (1.0 cm3) in the bilateral parietal regions. The peak areas for choline compounds, the sum of creatine and phosphocreatine, and N-acetyl-aspartate were calculated.

Results: Angiograms revealed approximately 50% reduction of vessel caliber for the right main cerebral arteries on Day 7. Magnetic resonance imaging revealed no apparent cerebral infarction, even in the spasm-side hemisphere. MRS revealed a significant (P < 0.05) reduction of the N-acetyl-aspartate/creatine and phosphocreatine ratio on Days 7 and 14 and a significant increase in the choline/creatine and phosphocreatine ratio on Day 7, in the spasm-side parietal region. In the sham-operated animals, there were no significant changes in these ratios in the bilateral parietal region on Days 7 and 14 after the operation.

Conclusion: The results suggested that the development of cerebral vasospasm after SAH caused ischemic injury in a subpopulation of neuronal cells, even when no apparent cerebral infarction was shown. Proton MRS may be useful to evaluate how neuronal cells are affected by the ischemic insult during development of vasospasm in clinical situations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / analysis
  • Brain / diagnostic imaging
  • Brain / metabolism*
  • Brain Ischemia / diagnostic imaging
  • Brain Ischemia / etiology
  • Brain Ischemia / metabolism
  • Cerebral Angiography
  • Cerebrovascular Circulation
  • Choline / analysis
  • Chronic Disease
  • Cognition Disorders / etiology
  • Creatinine / analysis
  • Energy Metabolism
  • Female
  • Ischemic Attack, Transient / diagnostic imaging
  • Ischemic Attack, Transient / etiology
  • Ischemic Attack, Transient / metabolism*
  • Macaca fascicularis
  • Magnetic Resonance Imaging / methods*
  • Neurons / metabolism
  • Neurons / pathology
  • Phosphocreatine / analysis
  • Subarachnoid Hemorrhage / complications
  • Subarachnoid Hemorrhage / metabolism


  • Phosphocreatine
  • Aspartic Acid
  • N-acetylaspartate
  • Creatinine
  • Choline