VE-cadherin antibody accelerates neutrophil recruitment in vivo

J Cell Sci. 1997 Mar:110 ( Pt 5):583-8. doi: 10.1242/jcs.110.5.583.


Neutrophils enter sites of inflammation by crossing the endothelial lining of the blood vessel wall. VE-cadherin is an endothelial specific, homophilic adhesion molecule located at the lateral cell surface. We have generated a monoclonal antibody against mouse VE-cadherin which inhibits electrical resistance of endothelial cell monolayers in vitro as well as aggregation of VE-cadherin transfected cells. In vivo, this antibody was found to increase vascular permeability and to accelerate the entry of neutrophils into chemically inflamed mouse peritoneum. Thus, VE-cadherin is essential for the integrity of the endothelial barrier in vivo. Our data suggest that opening of VE-cadherin mediated endothelial cell contacts may be a relevant step during neutrophil extravasation.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD
  • CHO Cells
  • Cadherins / genetics
  • Cadherins / immunology*
  • Capillary Permeability / immunology
  • Cell Adhesion / immunology
  • Cell Aggregation / immunology*
  • Cricetinae
  • Mice
  • Neutrophils / cytology*
  • Neutrophils / immunology
  • Rats
  • Rats, Inbred Lew
  • Transfection
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, CD
  • Cadherins
  • cadherin 5