Effects of nedocromil sodium on the oxidative burst of polymorphonuclear leukocytes: comparison with salbutamol

Drugs Exp Clin Res. 1997;23(1):33-8.

Abstract

The effects of nedocromil sodium and of salbutamol on the generation of oxygen-derived free radicals in human polymorphonuclear leukocytes (PMNs) were compared in vitro by the luminol-amplified-chemiluminescence (LACL) assay induced by both particulate (Candida albicans) and soluble formyl-methionyl-leucyl-phenylalanine (fMLP) stimulants. Inhibitory dose-effect linear regressions were observed from 10(-3) to 10(-8) M for nedocromil and salbutamol after a 3' period of incubation with either C. albicans or fMLP. There was a linear regression with nedocromil sodium after 30' incubation, but desensitization was observed with salbutamol after this longer period of incubation. The generation of oxygen-derived free radicals was significantly greater for asthmatic patients than for normal subjects; therefore antiasthmatic drugs with this inhibitory activity could be an extra pharmacological benefit in the treatment of asthmatic patients.

Publication types

  • Comparative Study

MeSH terms

  • Albuterol / pharmacology*
  • Analysis of Variance
  • Anti-Asthmatic Agents / pharmacology*
  • Bronchodilator Agents / pharmacology*
  • Candida albicans / drug effects
  • Candida albicans / metabolism
  • Dose-Response Relationship, Drug
  • Humans
  • Linear Models
  • Luminescent Measurements
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Nedocromil / pharmacology*
  • Neutrophils / cytology
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Reactive Oxygen Species
  • Respiratory Burst / drug effects*

Substances

  • Anti-Asthmatic Agents
  • Bronchodilator Agents
  • Reactive Oxygen Species
  • Nedocromil
  • N-Formylmethionine Leucyl-Phenylalanine
  • Albuterol