Reconstitution in vitro of the components and conditions required for the oxidative cross-linking of extracellular proteins in French bean (Phaseolus vulgaris L.)

FEBS Lett. 1997 Mar 17;405(1):95-8. doi: 10.1016/s0014-5793(97)00166-x.


Oxidative cross linking of three (hydroxy)proline-rich cell wall proteins, known to be immobilised during the elicitor-induced oxidative burst in French bean cells, was modelled using peroxidases with cysteine or H2O2. Further reconstitution of the homologous system was achieved with a 46 kDa bean cell-wall peroxidase using conditions known to appertain in cell walls prior to the immobilisation. Thus, cell wall alkalinisation and secretion of a reductant have been reconstituted by addition of apoplastic fluid to the wall proteins and the 46 kDa peroxidase with resultant cross-linking. This is the first demonstration of the oxidative cross-linking of cell wall glycoproteins without the addition of external H2O2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents / chemistry
  • Cross-Linking Reagents / pharmacology*
  • Cysteine / pharmacology
  • Fabaceae
  • Glycoproteins / chemistry
  • Glycoproteins / drug effects
  • Horseradish Peroxidase / pharmacology
  • Hydrogen Peroxide / pharmacology
  • Hydrogen-Ion Concentration
  • Oxidation-Reduction
  • Plant Proteins / chemistry*
  • Plant Proteins / drug effects
  • Plants, Medicinal


  • Cross-Linking Reagents
  • Glycoproteins
  • Plant Proteins
  • extensin protein, plant
  • Hydrogen Peroxide
  • Horseradish Peroxidase
  • Cysteine