Mac-1 (CD11b/CD18) is an oligodeoxynucleotide-binding protein

Nat Med. 1997 Apr;3(4):414-20. doi: 10.1038/nm0497-414.


We have studied the interactions of phosphodiester and phosphorothioate oligodeoxynucleotides with Mac-1 (CD11b/CD18; alpha M beta 2), a heparin-binding integrin found predominantly on the surface of polymorphonuclear leukocytes (PMNs), macrophages and natural killer cells. Binding of a homopolymer of thymidine occurred on both the alpha M and beta 2 subunits. Soluble fibrinogen, a natural ligand for Mac-1, was an excellent competitor of the binding of a phosphorothioate oligodeoxynucleotide to both TNF-alpha-activated and nonactivated PMNs. Upregulation of cell-surface Mac-1 expression increased cell-surface binding of oligodeoxynucleotides. Binding was inhibited by anti-Mac-1 monoclonal antibodies, and the increase in cell-surface binding was correlated with a three- to fourfold increase in internalization by PMNs. An oligodeoxynucleotide inhibited beta 2-dependent migration through Matrigel, but the production of reactive oxygen species in PMNs adherent to fibrinogen dramatically increased. Thus, our data demonstrate that Mac-1 is a cell-surface receptor for oligodeoxynucleotides that can mediate their internalization and that this binding may have important functional consequences.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / pharmacology
  • Binding, Competitive
  • CD18 Antigens / genetics
  • CD18 Antigens / immunology
  • CD18 Antigens / metabolism*
  • Chemotaxis, Leukocyte / drug effects
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Ligands
  • Macrophage-1 Antigen / genetics
  • Macrophage-1 Antigen / immunology
  • Macrophage-1 Antigen / metabolism*
  • Membrane Proteins*
  • Neutrophils / metabolism*
  • Oligodeoxyribonucleotides / metabolism*
  • Protein Binding / drug effects
  • Reactive Oxygen Species / metabolism
  • Receptors, Immunologic / metabolism
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Scavenger Receptors, Class B
  • Signal Transduction
  • Thionucleotides / metabolism
  • Up-Regulation


  • Antibodies, Monoclonal
  • CD18 Antigens
  • DNA-Binding Proteins
  • Ligands
  • Macrophage-1 Antigen
  • Membrane Proteins
  • Oligodeoxyribonucleotides
  • Reactive Oxygen Species
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class B
  • Thionucleotides