A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map

DNA Res. 1996 Dec 31;3(6):363-77. doi: 10.1093/dnares/3.6.363.


The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid beta-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping*
  • Escherichia coli / genetics*
  • Genes, Bacterial / genetics*
  • Multigene Family
  • Open Reading Frames
  • Operon
  • Plasmids / genetics
  • Replicon
  • Sequence Alignment
  • Sequence Analysis, DNA*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Siphoviridae / genetics

Associated data

  • GENBANK/D90763
  • GENBANK/D90764
  • GENBANK/D90765
  • GENBANK/D90766
  • GENBANK/D90768
  • GENBANK/D90769
  • GENBANK/D90771
  • GENBANK/D90772
  • GENBANK/D90773
  • GENBANK/D90774
  • GENBANK/D90775
  • GENBANK/D90776
  • GENBANK/D90777
  • GENBANK/D90778
  • GENBANK/D90779
  • GENBANK/D90781
  • GENBANK/D90782
  • GENBANK/D90783
  • GENBANK/D90784
  • GENBANK/D90785
  • GENBANK/D90787
  • GENBANK/D90788
  • GENBANK/D90790
  • GENBANK/D90791
  • GENBANK/D90792
  • GENBANK/D90793
  • GENBANK/D90795
  • GENBANK/D90797
  • GENBANK/D90798
  • GENBANK/D90799