T-DNA insertion mutagenesis in Arabidopsis: going back and forth

Trends Genet. 1997 Apr;13(4):152-6. doi: 10.1016/s0168-9525(97)01094-9.

Abstract

T-DNA insertion mutagenesis in Arabidopsis has been instrumental in advancing our knowledge of the physiology, biochemistry and development of plants. In just a few years plant biology, the late starter of molecular genetics, has placed itself at the forefront of science in large measure as a result of the more than 20000 T-DNA transformants that have been generated in Arabidopsis. To date, approximately 4000 mutants have been identified and more than 40 genes have been isolated via the T-DNA tag. The large population of transformed lines in now being subjected to a PCR-based reverse genetics strategy to identify new insertion mutants in sequences of interest.

Publication types

  • Review

MeSH terms

  • Arabidopsis / genetics*
  • DNA, Bacterial / genetics*
  • Genes, Plant
  • Mutagenesis, Insertional
  • Phenotype
  • Plants, Genetically Modified
  • Rhizobium / genetics

Substances

  • DNA, Bacterial
  • T-DNA