Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes

Nucleic Acids Res. 1977 Aug;4(8):2713-23. doi: 10.1093/nar/4.8.2713.

Abstract

In phytohemagglutinin stimulated human lymphocytes the time relationship was determined between induction of the parameters mentioned. The results indicate that the induction occurred in a specific sequence. Thus, a simultaneous increase in the activity of DNA polymerase and thymidinekinase occurred after 15 h of incubation with Phytohemagglutinin. Furthermore, this enhancement occurred 2 h before the expansion of the TTP and dCTP pools and 4 h before the expansion of the dATP and dGTP pools. The rate of [3H] deoxyguanosine incorporation into DNA increased simultaneously with the expansion of the TTP and dCTP pools.

MeSH terms

  • DNA / biosynthesis*
  • DNA-Directed DNA Polymerase / biosynthesis*
  • Deoxyribonucleotides / metabolism*
  • Enzyme Induction
  • Humans
  • Kinetics
  • Lectins*
  • Lymphocyte Activation*
  • Lymphocytes / enzymology
  • Lymphocytes / metabolism*
  • Thymidine Kinase / biosynthesis*

Substances

  • Deoxyribonucleotides
  • Lectins
  • DNA
  • Thymidine Kinase
  • DNA-Directed DNA Polymerase