Establishment and characterization of cloned CD4- CD8- alphabeta-T cell receptor (TCR)-bearing autoreactive T cells from autoimmune NZB x NZW F1 mice

Clin Exp Immunol. 1997 Apr;108(1):52-7. doi: 10.1046/j.1365-2249.1997.d01-971.x.

Abstract

Murine models such as NZB/W F1, NZB.H-2bm12 and MRL.lpr/lpr mice have provided greater insight into the pathogenic mechanisms of lupus. To understand further the roles of T cells and cytokines in the pathogenesis of murine lupus, 11 cloned anti-DNA antibodies augmenting autoreactive T cell lines were derived from NZB/W F1 mice. All these autoreactive cells responded to syngeneic splenic cells and helped syngeneic B cells to produce anti-DNA antibodies, especially the IgG antibody. Ten out of 11 autoreactive T cell lines expressed neither CD4 nor CD8 cell surface markers on their surface. In addition, the cytokine production pattern of these autoreactive T cell lines was predominantly of type 0 (Th0) or type 2 T helper cells (Th2). To further investigate the role of accessory molecules in the activation of these autoreactive T cell lines, expression of IL-2R and heat-stable antigen (HSA) on these autoreactive T cells was analysed. Results suggest that the HSA played a critical role in the activation and function of these double-negative cloned autoreactive T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Autoimmunity / immunology*
  • CD4 Antigens / analysis
  • CD8 Antigens / analysis
  • Clone Cells
  • Cytokines / analysis
  • Female
  • Histocompatibility Antigens Class II / immunology
  • Lupus Vulgaris / immunology
  • Male
  • Mice
  • Phenotype
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • Receptors, Interleukin-2 / analysis
  • T-Lymphocytes / classification
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*

Substances

  • Antibodies
  • CD4 Antigens
  • CD8 Antigens
  • Cytokines
  • Histocompatibility Antigens Class II
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Interleukin-2