Refractive-index detection by interferometric backscatter in packed-capillary high-performance liquid chromatography

J Chromatogr A. 1997 Feb 21;762(1-2):219-25. doi: 10.1016/s0021-9673(96)00852-7.


A simple, inexpensive, micro-volume refractive index detector has been applied to packed capillary high-performance liquid chromatography. Nanoliter detector volumes produced by a unique optical train, based on interferometric backscatter, allows for universal solute detection at the picogram level. System utility is demonstrated by reversed-phase separation of a test mixture containing ppm levels of NaCl, phenol, ethyl benzene and toluene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Refractometry / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity