nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13

Hum Genet. 1997 Apr;99(4):550-7. doi: 10.1007/s004390050405.


A novel human nm23/nucleoside diphosphate (NDP) kinase gene, called nm23-H4, was identified by screening a human stomach cDNA library with a probe generated by amplification by reverse transcription-polymerase chain reaction. The primers were designed from publicly available database cDNA sequences selected according to their homology to the human nn23-H1 putative metastasis suppressor gene. The full-length cDNA sequence predicts a 187 amino acid protein possessing the region homologous to NDP kinases with all residues crucial for nucleotide binding and catalysis, strongly suggesting that Nm23-H4 possesses NDP kinase activity. It shares 56, 55 and 60% identity with Nm23-H1, Nm23-H2 and DR-Nm23, respectively, the other human Nm23 proteins isolated so far. Compared with these proteins, Nm23-H4 contains an additional NH2-terminal region that is rich in positively charged residues and could indicate routing to mitochondria. The nm23-H4 gene has been localised to human chromosomal band 16p13.3. The corresponding 1.2 kb mRNA is widely distributed and expressed in a tissue-dependent manner, being found at very high levels in prostate, heart, liver, small intestine and skeletal muscle tissues and in low amounts in the brain and in blood leucocytes. Nm23-H4 naturally possesses the Pro-Ser substitution equivalent to the K-pn mutation (P97S) of Drosophila.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 16*
  • DNA, Complementary
  • Humans
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins*
  • Multigene Family
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside Diphosphate Kinase D
  • Nucleoside-Diphosphate Kinase / genetics*
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*


  • DNA, Complementary
  • NM23 Nucleoside Diphosphate Kinases
  • Transcription Factors
  • NME1 protein, human
  • NME4 protein, human
  • Nucleoside Diphosphate Kinase D
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins

Associated data

  • GENBANK/Y07604