delta-Thalassemia reduces the expected HbA2 percentage, altering the normal as well as the beta-thalassemia trait phenotype. An attempt to elucidate the molecular basis of delta-thalassemia in the Greek population, revealed two cases with unknown molecular defects that presented low levels of HbA2 (about 1.5%). DNA sequence analysis of delta-globin gene identified two "novel" mutations in the coding regions of the gene; the cd11 (GTC-->GGC) resulting in the substitution of valine for glycine (:HbA2-Pylos) and the cd85(TTT-->TCT) resulting in the substitution of phenylalanine for serine (:HbA2-Etolia). Because these mutations are localized at the helical positions A8 and F1 of the HbA2 respectively, they potentially cause molecular instability of the tetramer, thus leading to reduced HbA2 percentage.