Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12

FEMS Microbiol Lett. 1997 Apr 1;149(1):115-20. doi: 10.1111/j.1574-6968.1997.tb10317.x.

Abstract

The product controlling colony form variation and autoaggregation in Escherichia coli K-12 (the flu gene product) has been identified as the phase-variable, bipartite, outer membrane protein, termed antigen 43 (Ag43). Identification is based: (i) on complete correlation in authentic flu variants between colony morphology/autoaggregation and Ag43 expression as determined by colony and Western immunoblotting and immunofluorescence microscopy; and (ii) on the use of a specific probe to map the gene encoding Ag43 to a position (min 43) on the E. coli chromosome previously established for flu.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibody Specificity
  • Antigens, Bacterial / genetics*
  • Antigens, Bacterial / immunology
  • Antigens, Bacterial / isolation & purification
  • Bacterial Adhesion / physiology
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / immunology
  • Bacterial Outer Membrane Proteins / isolation & purification
  • Blotting, Southern
  • Blotting, Western
  • DNA Probes
  • Escherichia coli / chemistry*
  • Escherichia coli / cytology*
  • Escherichia coli / growth & development
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Bacterial / physiology
  • Immunoblotting
  • Molecular Sequence Data
  • Polymerase Chain Reaction

Substances

  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • DNA Probes