Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45

Biochim Biophys Acta. 1997 Apr 3;1325(1):8-12. doi: 10.1016/s0005-2736(97)00014-x.


We have identified the rat homologue (rvps45) of the yeast vps45 protein, a member of the Sec1 family of proteins involved in intracellular vesicle trafficking. Sequence analysis of the full-length rvps45 cDNA obtained from a rat brain library predicts a protein of 570 amino acids which shares 36% identity with the yeast vps45 protein. The sequence shows less homology with other mammalian Sec1 family proteins. Northern blotting identified a 2.3 kb mRNA highly expressed in brain and testis. RT-PCR analysis showed that the rvps45 gene product is expressed throughout the brain. The homology of this protein with the yeast vps45 together with its high expression in brain suggests a role for rvps45 in transport from the Golgi complex to synaptic vesicles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry / genetics
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • Fungal Proteins / genetics
  • Intracellular Membranes / chemistry*
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Rats
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Vesicular Transport Proteins*


  • Carrier Proteins
  • Fungal Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Saccharomyces cerevisiae Proteins
  • VPS45 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Vps45 protein, rat

Associated data

  • GENBANK/U81160