Genomic organization of the human excitatory amino acid transporter gene GLT-1

Neuroreport. 1997 Feb 10;8(3):775-7. doi: 10.1097/00001756-199702100-00039.


We present the genomic structure of the human glutamate transporter GLT-1 coding region, the intronic sequences adjacent to the exons, and oligonucleotide primer sequences for single strand conformational analysis. The exon-intron boundaries were determined using long-distance PCR and direct sequencing. The human GLT-1 coding region is composed of 10 exons spanning > 50 kb of genomic DNA. The exons range from 127 to 251 bp in length. The intron lengths vary considerably from 2.2 kb to > 15 kb. These data provide the basis for implementing a comprehensive screen for genetic alterations in the human GLT-1 gene using genomic DNA as a template.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / biosynthesis
  • ATP-Binding Cassette Transporters / genetics*
  • Amino Acid Transport System X-AG
  • Base Sequence
  • Biological Transport
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • DNA Primers
  • Exons
  • Genome, Human
  • Humans
  • Introns
  • Polymerase Chain Reaction


  • ATP-Binding Cassette Transporters
  • Amino Acid Transport System X-AG
  • DNA Primers