Identification of a Methyl Jasmonate-Responsive Region in the Promoter of a Lipoxygenase 1 Gene Expressed in Barley Grain

Plant J. 1997 Mar;11(3):513-23. doi: 10.1046/j.1365-313x.1997.11030513.x.

Abstract

A genomic DNA fragment was isolated containing 5' upstream sequences and part of the open reading frame corresponding to the lipoxygenase 1 cDNA (LoxA) expressed in barley grains during development and germination. Lox1 transcription was shown to be methyl jasmonate (MeJA)- and wound-inducible in leaves, but Lox1 transcripts were not detected in mildew-infected leaves, although this is a commonly observed response to pathogenic attack in various plants. Transient gene expression assays were used to identify a promoter region involved in MeJA-responsive expression. Analysis of 5' and 3' promoter deletions indicated that sequences between -363 and -294 conferred MeJA-responsive expression. Deletions/replacements covering this part of the promoter further defined a MeJA-responsive region between -331 and -291. Insertion of the region -328 to -293 into the constitutive CaMV 35S promoter conferred MeJA-responsive expression. The 36 bp fragment contains the motif TGACG as inverted repeats, which has been previously identified as a binding site for bZIP transactivating factors. Site-directed mutagenesis on these TGACG motifs abolished MeJA-responsive expression, clearly identifying them as MeJA-responsive elements. Sequence comparisons found no similar motif in other characterized promoters of MeJA-inducible genes, but suggested a common spatial structure which may serve as a binding site for transacting factors involved in the MeJA signal transduction pathway.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Acetates / pharmacology*
  • Base Sequence
  • Cloning, Molecular
  • Cyclopentanes / pharmacology*
  • Enzyme Induction
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Hordeum / enzymology*
  • Hordeum / genetics
  • Hordeum / growth & development
  • Lipoxygenase / biosynthesis*
  • Lipoxygenase / genetics*
  • Molecular Sequence Data
  • Open Reading Frames
  • Oxylipins
  • Plant Growth Regulators / pharmacology
  • Promoter Regions, Genetic*
  • Recombinant Fusion Proteins / biosynthesis
  • Restriction Mapping

Substances

  • Acetates
  • Cyclopentanes
  • Oxylipins
  • Plant Growth Regulators
  • Recombinant Fusion Proteins
  • Abscisic Acid
  • methyl jasmonate
  • Lipoxygenase

Associated data

  • GENBANK/L37358
  • GENBANK/L37359
  • GENBANK/U83904