Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 25 (9), 1782-7

A Physico-Chemical Study of Triple Helix Formation by an Oligodeoxythymidylate With N3'--> P5' Phosphoramidate Linkages

Affiliations

A Physico-Chemical Study of Triple Helix Formation by an Oligodeoxythymidylate With N3'--> P5' Phosphoramidate Linkages

B Zhou-Sun et al. Nucleic Acids Res.

Abstract

Non-denaturing gel retardation assay, DNA melting experiments and FTIR spectroscopy were used to characterize the triple helix formed by a 15mer 2'-deoxythymidylate with N3'-->P5'phosphoramidate linkages with its target sequence. The results indicate that: (i) the pentadecadeoxythymidylate with phosphoramidate linkages [dT15(np)] is highly potent to form a triple helix with a dT15*dA15target duplex through Hoogsteenbase-pairing; (ii) it forms a dT15(np)*dA15xdT15(np) triplex with the single-stranded oligo-2'-deoxyadenylate (dA15) without detectable double-helical intermediate; (iii) it does not only form a triple helix on the dT15*dA15target duplex, but also partially displaces the dT15 strand from the dT15*dA15duplex to form a dT15(np)*dA15xdT15(np) complex.

Similar articles

See all similar articles

Cited by 2 articles

References

    1. Biopolymers. 1970;9(9):1059-77 - PubMed
    1. Eur J Cancer. 1994;30A(11):1721-6 - PubMed
    1. Science. 1987 Oct 30;238(4827):645-50 - PubMed
    1. Biochim Biophys Acta. 1990 Jun 21;1049(2):99-125 - PubMed
    1. Nucleic Acids Res. 1990 Oct 11;18(19):5743-50 - PubMed

MeSH terms

LinkOut - more resources

Feedback