A wat1 mutant of fission yeast is defective in cell morphology

Mol Gen Genet. 1997 Mar 26;254(2):127-38. doi: 10.1007/s004380050400.

Abstract

The organization of the actin cytoskeleton plays an integral role in cell morphogenesis of all eukaryotes. We have isolated a temperature-sensitive mutant in Schizosaccharomyces pombe, wat1-1, in which acting patches are delocalized, resulting in an elliptically shaped cell phenotype. Molecular cloning and DNA sequencing of wat1+ showed that the gene encodes a 314 residue protein containing WD-40 repeats. Cells lacking wat1+ are slow growing but viable at 25 degrees C and temperature-sensitive for growth above 33 degrees C. At restrictive temperature, wat1-d strains are phenotypically indistinguishable from wat1-1. When combined with a deletion for the wat1+ gene, cdc mutants failed to elongate at restrictive temperature and exhibited alterations in actin patch localization. This analysis suggests that wat1+ is required directly or indirectly for polarized cell growth in S. pombe. Wat1p and a functional, epitope-tagged, version of Wat1p can be overproduced without inducing alterations in cell morphology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics*
  • Genes, Fungal
  • Molecular Sequence Data
  • Mutation*
  • Phenotype
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid
  • Temperature

Substances

  • Actins
  • DNA, Fungal
  • Fungal Proteins
  • Pop3 protein, S pombe
  • Schizosaccharomyces pombe Proteins

Associated data

  • GENBANK/X77114