Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 405 (3), 305-11

RNA Polymerase III Interferes With Ty3 Integration

Affiliations

RNA Polymerase III Interferes With Ty3 Integration

C M Connolly et al. FEBS Lett.

Abstract

Ty3, a gypsylike retrotransposon of budding yeast, integrates at the transcription initiation site of genes transcribed by RNA polymerase III (pol III). It was previously shown that integration in vitro requires intact promoter elements and the pol III transcription factors TFIIIB and TFIIIC. In order to test the effect of pol III on integration, increasing amounts of a pol III-containing fraction were added to Ty3 in vitro integration reactions. The pol III-containing fraction was inhibitory to integration. These results are consistent with a model where the Ty3 integration complex and pol III recognize similar features of the stable transcription complex and compete with each other for access to the transcription initiation site.

Similar articles

See all similar articles

Cited by 11 articles

See all "Cited by" articles

Publication types

MeSH terms

Feedback