Sensing by intrahepatic muscarinic nerves of a portal-arterial glucose concentration gradient as a signal for insulin-dependent glucose uptake in the perfused rat liver

FEBS Lett. 1997 Apr 7;406(1-2):119-22. doi: 10.1016/s0014-5793(97)00254-8.


In vivo, insulin increases net hepatic glucose uptake efficiently only in the presence of a portal-arterial glucose gradient. In isolated perfused rat livers supplied with a glucose gradient (portal 10 mM/arterial 5 mM) insulin-induced glucose uptake was blocked by atropine; in livers not supplied with the gradient (portal = arterial 5 mM) insulin-dependent glucose uptake was elicited by acetylcholine. Apparently, the gradient was sensed and transformed into a metabolic signal by intrahepatic nerves, releasing acetylcholine to muscarinic receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glucose / metabolism*
  • Insulin / pharmacology*
  • Liver / blood supply
  • Liver / drug effects
  • Liver / injuries
  • Liver / metabolism*
  • Male
  • Perfusion
  • Rats
  • Rats, Wistar
  • Receptors, Muscarinic / physiology*


  • Insulin
  • Receptors, Muscarinic
  • Glucose