Abstract
Corticosteroids modulate the expression and/or functions of several serotonin (5-hydroxytryptamine; 5-HT) receptors. Conversely, analyses of the effects of corticosteroids upon 5-HT reuptake systems have been scarce and contradictory. Herein, the diurnal rhythm of midbrain [3H]citalopram binding to 5-HT transporters was analysed in sham and 11 day adrenalectomised rats. In addition, adrenalectomised rats were either complemented or not with corticosterone pellets (12.5-200 mg). Analyses of body weight increases and plasma adrenocorticotropic and corticosterone levels indicated that the protocol allowed the stimulation of mineralocorticoid receptors (MRs; 12.5 mg pellets) or the stimulation of both MRs and glucocorticoid receptors (GRs; 50-200 mg pellets). However, besides the observation of a slight, but significant diurnal (corticosteroid-independent) rhythm in 5-HT transporter binding (morning > evening), it was found that neither adrenalectomy nor corticosteroid receptor stimulation affected midbrain [3H]citalopram binding.
MeSH terms
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Adrenalectomy*
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Adrenocorticotropic Hormone / blood
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Body Weight
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Carrier Proteins / metabolism
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Circadian Rhythm / drug effects
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Citalopram / metabolism
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Citalopram / pharmacology*
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Corticosterone / pharmacology*
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Hypothalamo-Hypophyseal System / physiology
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Male
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Membrane Glycoproteins / metabolism
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Membrane Transport Proteins*
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Mesencephalon / chemistry*
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Mesencephalon / metabolism
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Nerve Tissue Proteins*
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Organ Size
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Pituitary-Adrenal System / physiology
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Protein Binding / physiology
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Rats
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Rats, Wistar
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Selective Serotonin Reuptake Inhibitors / metabolism
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Selective Serotonin Reuptake Inhibitors / pharmacology*
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Serotonin / metabolism
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Serotonin Plasma Membrane Transport Proteins
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Thymus Gland / drug effects
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Tritium
Substances
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Anti-Inflammatory Agents
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Carrier Proteins
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Membrane Glycoproteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Serotonin Plasma Membrane Transport Proteins
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Serotonin Uptake Inhibitors
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Slc6a4 protein, rat
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Citalopram
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Tritium
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Serotonin
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Adrenocorticotropic Hormone
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Corticosterone